Overexpression of the HspL Promotes Agrobacterium tumefaciens Virulence in Arabidopsis Under Heat Shock Conditions.
Identifieur interne : 000342 ( Main/Exploration ); précédent : 000341; suivant : 000343Overexpression of the HspL Promotes Agrobacterium tumefaciens Virulence in Arabidopsis Under Heat Shock Conditions.
Auteurs : Hau-Hsuan Hwang ; Yin-Tzu Liu ; Si-Chi Huang ; Chin-Yi Tung ; Fan-Chen Huang ; Yun-Long Tsai ; Tun-Fang Cheng ; Erh-Min LaiSource :
- Phytopathology [ 0031-949X ] ; 2015.
Descripteurs français
- KwdFr :
- ADN bactérien (génétique), Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (pathogénicité), Agrobacterium tumefaciens (physiologie), Arabidopsis (microbiologie), Facteurs de virulence (génétique), Maladies des plantes (microbiologie), Plasmides (génétique), Protéines bactériennes (génétique), Protéines du choc thermique (génétique), Racines de plante (microbiologie), Régulation de l'expression des gènes bactériens (MeSH), Stress physiologique (MeSH), Température élevée (MeSH), Tumeurs végétales (MeSH), Virulence (MeSH).
- MESH :
- génétique : ADN bactérien, Agrobacterium tumefaciens, Facteurs de virulence, Plasmides, Protéines bactériennes, Protéines du choc thermique.
- microbiologie : Arabidopsis, Maladies des plantes, Racines de plante.
- pathogénicité : Agrobacterium tumefaciens.
- physiologie : Agrobacterium tumefaciens.
- Régulation de l'expression des gènes bactériens, Stress physiologique, Température élevée, Tumeurs végétales, Virulence.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (pathogenicity), Agrobacterium tumefaciens (physiology), Arabidopsis (microbiology), Bacterial Proteins (genetics), DNA, Bacterial (genetics), Gene Expression Regulation, Bacterial (MeSH), Heat-Shock Proteins (genetics), Hot Temperature (MeSH), Plant Diseases (microbiology), Plant Roots (microbiology), Plant Tumors (MeSH), Plasmids (genetics), Stress, Physiological (MeSH), Virulence (MeSH), Virulence Factors (genetics).
- MESH :
- chemical , genetics : Bacterial Proteins, DNA, Bacterial, Heat-Shock Proteins, Virulence Factors.
- genetics : Agrobacterium tumefaciens, Plasmids.
- microbiology : Arabidopsis, Plant Diseases, Plant Roots.
- pathogenicity : Agrobacterium tumefaciens.
- physiology : Agrobacterium tumefaciens.
- Gene Expression Regulation, Bacterial, Hot Temperature, Plant Tumors, Stress, Physiological, Virulence.
Abstract
Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.
DOI: 10.1094/PHYTO-05-14-0133-R
PubMed: 25163013
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Arabidopsis Under Heat Shock Conditions.</title><author><name sortKey="Hwang, Hau Hsuan" sort="Hwang, Hau Hsuan" uniqKey="Hwang H" first="Hau-Hsuan" last="Hwang">Hau-Hsuan Hwang</name></author><author><name sortKey="Liu, Yin Tzu" sort="Liu, Yin Tzu" uniqKey="Liu Y" first="Yin-Tzu" last="Liu">Yin-Tzu Liu</name></author><author><name sortKey="Huang, Si Chi" sort="Huang, Si Chi" uniqKey="Huang S" first="Si-Chi" last="Huang">Si-Chi Huang</name></author><author><name sortKey="Tung, Chin Yi" sort="Tung, Chin Yi" uniqKey="Tung C" first="Chin-Yi" last="Tung">Chin-Yi Tung</name></author><author><name sortKey="Huang, Fan Chen" sort="Huang, Fan Chen" uniqKey="Huang F" first="Fan-Chen" last="Huang">Fan-Chen Huang</name></author><author><name sortKey="Tsai, Yun Long" sort="Tsai, Yun Long" uniqKey="Tsai Y" first="Yun-Long" last="Tsai">Yun-Long Tsai</name></author><author><name sortKey="Cheng, Tun Fang" sort="Cheng, Tun Fang" uniqKey="Cheng T" first="Tun-Fang" last="Cheng">Tun-Fang Cheng</name></author><author><name sortKey="Lai, Erh Min" sort="Lai, Erh Min" uniqKey="Lai E" first="Erh-Min" last="Lai">Erh-Min Lai</name></author></analytic><series><title level="j">Phytopathology</title><idno type="ISSN">0031-949X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (pathogenicity)</term><term>Agrobacterium tumefaciens (physiology)</term><term>Arabidopsis (microbiology)</term><term>Bacterial Proteins (genetics)</term><term>DNA, Bacterial (genetics)</term><term>Gene Expression Regulation, Bacterial (MeSH)</term><term>Heat-Shock Proteins (genetics)</term><term>Hot Temperature (MeSH)</term><term>Plant Diseases (microbiology)</term><term>Plant Roots (microbiology)</term><term>Plant Tumors (MeSH)</term><term>Plasmids (genetics)</term><term>Stress, Physiological (MeSH)</term><term>Virulence (MeSH)</term><term>Virulence Factors (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN bactérien (génétique)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (pathogénicité)</term><term>Agrobacterium tumefaciens (physiologie)</term><term>Arabidopsis (microbiologie)</term><term>Facteurs de virulence (génétique)</term><term>Maladies des plantes (microbiologie)</term><term>Plasmides (génétique)</term><term>Protéines bactériennes (génétique)</term><term>Protéines du choc thermique (génétique)</term><term>Racines de plante (microbiologie)</term><term>Régulation de l'expression des gènes bactériens (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Température élevée (MeSH)</term><term>Tumeurs végétales (MeSH)</term><term>Virulence (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>DNA, Bacterial</term><term>Heat-Shock Proteins</term><term>Virulence Factors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN bactérien</term><term>Agrobacterium tumefaciens</term><term>Facteurs de virulence</term><term>Plasmides</term><term>Protéines bactériennes</term><term>Protéines du choc thermique</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Arabidopsis</term><term>Maladies des plantes</term><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Arabidopsis</term><term>Plant Diseases</term><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Bacterial</term><term>Hot Temperature</term><term>Plant Tumors</term><term>Stress, Physiological</term><term>Virulence</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Régulation de l'expression des gènes bactériens</term><term>Stress physiologique</term><term>Température élevée</term><term>Tumeurs végétales</term><term>Virulence</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">25163013</PMID><DateCompleted><Year>2015</Year><Month>08</Month><Day>06</Day></DateCompleted><DateRevised><Year>2015</Year><Month>01</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0031-949X</ISSN><JournalIssue CitedMedium="Print"><Volume>105</Volume><Issue>2</Issue><PubDate><Year>2015</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Phytopathology</Title><ISOAbbreviation>Phytopathology</ISOAbbreviation></Journal><ArticleTitle>Overexpression of the HspL Promotes Agrobacterium tumefaciens Virulence in Arabidopsis Under Heat Shock Conditions.</ArticleTitle><Pagination><MedlinePgn>160-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PHYTO-05-14-0133-R</ELocationID><Abstract><AbstractText>Agrobacterium tumefaciens transfers a specific DNA fragment from the resident tumor-inducing (Ti) plasmid and effector virulence (Vir) proteins to plant cells during infection. A. tumefaciens VirB1-11 and VirD4 proteins assemble as the type IV secretion system (T4SS), which mediates transfer of the T-DNA and effector Vir protein into plant cells, thus resulting in crown gall disease in plants. Previous studies revealed that an α-crystallin-type, small heat-shock protein (HspL) is a more effective VirB8 chaperone than three other small heat-shock proteins (HspC, HspAT1, and HspAT2). Additionally, HspL contributes to efficient T4SS-mediated DNA transfer and tumorigenesis under room-temperature growth. In this study, we aimed to characterize the impact of HspL on Agrobacterium-mediated transformation efficiency under heat-shock treatment. During heat shock, transient transformation efficiency and VirB8 protein accumulation were lower in the hspL deletion mutant than in the wild type. Overexpression of HspL in A. tumefaciens enhanced the transient transformation efficiency in root explants of both susceptible and recalcitrant Arabidopsis ecotypes. In addition, the reduced transient transformation efficiency during heat stress was recovered by overexpression of HspL in A. tumefaciens. HspL may help maintain VirB8 homeostasis and elevate Agrobacterium-mediated transformation efficiency under both heat-shock and nonheat-shock growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Hau-Hsuan</ForeName><Initials>HH</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yin-Tzu</ForeName><Initials>YT</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Si-Chi</ForeName><Initials>SC</Initials></Author><Author ValidYN="Y"><LastName>Tung</LastName><ForeName>Chin-Yi</ForeName><Initials>CY</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Fan-Chen</ForeName><Initials>FC</Initials></Author><Author ValidYN="Y"><LastName>Tsai</LastName><ForeName>Yun-Long</ForeName><Initials>YL</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Tun-Fang</ForeName><Initials>TF</Initials></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Erh-Min</ForeName><Initials>EM</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Phytopathology</MedlineTA><NlmUniqueID>9427222</NlmUniqueID><ISSNLinking>0031-949X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004269">DNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006360">Heat-Shock Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance 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Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015964" MajorTopicYN="N">Gene Expression Regulation, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006360" MajorTopicYN="N">Heat-Shock Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006358" MajorTopicYN="N">Hot Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010941" MajorTopicYN="N">Plant Tumors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010957" MajorTopicYN="N">Plasmids</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037521" MajorTopicYN="N">Virulence Factors</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2014</Year><Month>8</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2014</Year><Month>8</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2015</Year><Month>8</Month><Day>8</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">25163013</ArticleId><ArticleId IdType="doi">10.1094/PHYTO-05-14-0133-R</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Cheng, Tun Fang" sort="Cheng, Tun Fang" uniqKey="Cheng T" first="Tun-Fang" last="Cheng">Tun-Fang Cheng</name><name sortKey="Huang, Fan Chen" sort="Huang, Fan Chen" uniqKey="Huang F" first="Fan-Chen" last="Huang">Fan-Chen Huang</name><name sortKey="Huang, Si Chi" sort="Huang, Si Chi" uniqKey="Huang S" first="Si-Chi" last="Huang">Si-Chi Huang</name><name sortKey="Hwang, Hau Hsuan" sort="Hwang, Hau Hsuan" uniqKey="Hwang H" first="Hau-Hsuan" last="Hwang">Hau-Hsuan Hwang</name><name sortKey="Lai, Erh Min" sort="Lai, Erh Min" uniqKey="Lai E" first="Erh-Min" last="Lai">Erh-Min Lai</name><name sortKey="Liu, Yin Tzu" sort="Liu, Yin Tzu" uniqKey="Liu Y" first="Yin-Tzu" last="Liu">Yin-Tzu Liu</name><name sortKey="Tsai, Yun Long" sort="Tsai, Yun Long" uniqKey="Tsai Y" first="Yun-Long" last="Tsai">Yun-Long Tsai</name><name sortKey="Tung, Chin Yi" sort="Tung, Chin Yi" uniqKey="Tung C" first="Chin-Yi" last="Tung">Chin-Yi Tung</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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